DEVELOPMENT OF AN AUTOMATED IMMUNOTURBIDIMETRIC FERRITIN ASSAY PDF

Such assays are suitable for the measurement of proteins at concentrations >5– 10 mg/L. .. Development of an automated immunoturbidimetric ferritin assay. May 3, Different laboratory methods are used to quantify ferritin . assays and automated equipment developments, and in routine laboratory assays and Seco M. Development of an automated immunoturbidimetric ferritin assay. J. Colloid. Interface Sci. (2): – 3. Borque, L., Rus, A., Bellod, L., and Seco, M.L. Development of an automated immunoturbidimetric ferritin assay.

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We monitored the reaction kinetics at pH values of 6.

Among the many commercially available assays for CRP, the most common use fluid-phase or particle-enhanced turbidimetric or nephelometric procedures 7.

A new method of measuring C-reactive protein, with a low limit of detection, suitable for risk assessment of coronary heart disease. Jadsamount of antibody adsorbed; Jinamount of antibody added. Carboxilated latex particles nm in diameter were covalently coupled with immunopurified F ab’ 2 fragments of anti-ferritin IgG antibodies. The microparticle reagents were prepared according to a previously published method by the carbodiimide [1-ethyl 3-dimethylamino-propyl carbodiimide chloride] procedure Biomarker Menu Search Search by Keyword: Posttreatment with additives BSA, surfactants has been proposed 13 14although it reduces IgG charge and reactivity.

We suggest an alternative approach that uses a small amount of immunopurified antibody and stabilizes the reagent by saturation of free surface with BSA in a two-step sequential covalent procedure. Biological or Clinical Significance: J Clin Immunoassay ; CRP is a prognostic risk factor for coronary heart disease in both patients with angina stable or not and in apparently healthy subjects 2and distinguishes rapidly destructive vs slowly progressive osteoarthritis 3.

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The effect of varying the particle size on a reagent with a constant charge of 0. Clinical Chemistry Nov46 11.

Development of an automated immunoturbidimetric ferritin assay.

We conclude that this latex turbidimetric immunochemical procedure is simple, rapid, has a good analytical and operational performance on the Falcor analyzer and is well suited for the measurement of ferritin concentration in human serum. Evaluation of an improved immunonephelometric assay for C-reactive protein [Abstract]. C-reactive protein and bacterial infection in preterm infants.

Samples with CRP values above the upper limit of the calibration curve can be reanalyzed after automated or manual sample dilution. Colloidal stability and immunoreactivity. Clin Chem ; 42 Suppl 6: Clin Chem ; Clin Chem ; 45 Suppl 6: A rapid and sensitive automated light scattering immunoassay for serum C-reactive protein and the definition of a reference range in healthy blood donors.

Nevertheless, these results can be influenced by the sensitivity of the methodology used for CRP quantification. Ann Clin Biochem ; Coverage was calculated by measuring the uncoupled IgG present in the supernatants of the first centrifugation, after filtering with a Nucleopore polycarbonate filter pore diameter, 0. Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency.

We found that at any given working dilution of microparticles studied, the initial absorbance decreases with increasing wavelength and increases devleopment microparticle diameter, according to the light-scattering theory. Variability in the measurement of C-reactive protein in healthy subjects: We chose this microparticle devrlopment, with a coverage of 0.

The covalently coupled isotherms of IgG anti-CRP on carboxyl-modified polystyrene particles nm diameter are shown in Fig. Test Directory Entire Site. J Biomater Sci Polym Ed ; 3: There were no interferences from bilirubin, hemoglobin or rheumatoid factors.

Immunoturbidimetic methods of developmwnt CRP have lower limits of detection one-tenth those of earlier methods 8 9. C-Reactive protein CRP is a marker of acute phase response to inflammation that increases rapidly within hours of disease onset.

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Development of an automated immunoturbidimetric ferritin assay.

For the immunopurified anti-CRP antibodies, we used a modified procedure consisting of two sequential covalent coupling steps. It allows lower antibody coverage on the microparticle reagent and provides highly sensitive and robust particle-enhanced turbidimetric immunoassay for serum CRP.

Colloidal ffrritin coated with polyclonal IgG usually are not very stable, mainly because of the high antibody coverage needed to obtain good sensitivities 3.

View inline View popup. Increased serum C-reactive protein levels by immunonephelometry in patients with rapidly destructive hip osteoarthritis. Jump to section Article Acknowledgments References. We then selected 0.

The procedure has been automated using the Falcor analyzer. J Immunol Methods ; Principle of Test Method: Parameter values abcorrelation coefficients rand confidence regions were as follows: Table of Contents Index by author. Download this assay page in pdf format.

Evaluation of four automated high-sensitivity C-reactive protein methods: Ferritin is protein that has a central role in the storage of iron. Results obtained with the present procedure correlated well with those obtained by a nephelometric procedure and with those ferritjn by an RIA. Clin Chem Lab Med ; Thomas C, Thomas L. Show more Technical Briefs.

A microparticle size of nm and a wavelength of nm were selected as the best choice because kmmunoturbidimetric the high slope and wide dynamic range showed.

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